Test Details: This test aids in detection of folate levels in the RBC. Folate, with vitamin B12, is essential for DNA synthesis, required for normal red blood cell maturation. Folate and vitamin B12 deficiency impair DNA synthesis, causing macrocytic anemias. Thus this test is utilized to identify folate deficiency, monitor therapy with folate, evaluate megaloblastic and macrocytic anemia. Also red blood cell folate concentration is considered the most reliable indicator of folate status, hence preferred by several clinicians over serum folate.